CD4 抗体
Quick Overview for CD4 抗体 (ABIN118487)
抗原
See all CD4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognises CD4. Epitope mapping studies have shown that antibodies, produced by clone RPA-T4, recognise an epitope within domain 1, of the extracellular region, of the CD4 Molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation. We recommend the use of SM1058LE for this purpose.
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纯化方法
- Affinity chromatography on Protein G
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免疫原
- Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
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亚型
- IgG1
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anti-CD4 (CD4) (AA 385-457) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 犬, Pig, 兔, Cat, Cow, 绵羊, 豚鼠, 山羊, 马, Avian, 驴, Hamster WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 适用: 人 FACS, WB, MS 宿主: 小鼠 Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) (AA 301-400) antibodyCD4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) (AA 26-200) antibodyCD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC
anti-CD4 (CD4) (N-Term) antibody (PE)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE
anti-CD4 (CD4) antibody (PE)CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE
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应用备注
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Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on frozen sections: 1/10 - 1/100. Recommended positive control: Human lymphatic tissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD4
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别名
- CD4
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背景
- CD4 is a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
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基因ID
- 920
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NCBI登录号
- NP_000607
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UniProt
- P01730
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途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
抗原
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